WebTubular J-aggregates of a new thiacarbocyanine Cy5 dye for the far-red spectral region – a spectroscopic and cryo-transmission electron microscopy study - Physical Chemistry … Because they yield brighter and more stable fluorescence, cyanines can advantageously replace conventional dyes such as fluorescein and rhodamines. • Cy3 and Cy5 are the most popular, used typically combined for 2 colors detection. Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while …
Is Cy5 hydrophobic? AAT Bioquest
WebAlthough the photoconversion of far-red fluorophores poses potential complications for multicolor imaging, the facile dye-to-dye photoconversion of AF647 or Cy5 can be exploited to obtain high-density maps of single-molecule trajectories (Figure 5). Current methods for single-molecule tracking in a living cell rely almost invariably on ... WebCy5 synonyms, Cy5 pronunciation, Cy5 translation, English dictionary definition of Cy5. n. Any of various blue dyes, used to sensitize photographic emulsions to a greater range of … carafe for keurig coffee maker
Protein Labeling Kits User Guide (For Alexa Fluor, Pacific Blue ...
WebCyanine5.5 N-hydroxysuccinimide ester Biological description Far-red / near-infrared emitting fluorescent dye for labeling of amino-groups in peptides, proteins, and oligonucleotides. Analog of Cy5.5 ® NHS ester. Ideal for fluorescence measurements where background fluorescence is a concern. WebSulfo-Cyanine5.5 azide is a derivative of water soluble far red emitting fluorescent cyanine dye. The azide group can be conjugated by either copper-catalyzed or copper free Click chemistry. The reagent possesses high aqueous solubility and hydrophilicity, and recommended for the labeling of biomolecules in aqueous environment. WebTandem dyes (eg PE-Cy5) are more problematic due to variation in chemical conjugation. The use of the same batch for tandem conjugates is recommended. As a general rule, compensate with the fluorochromes from the far-red end of the spectrum (higher wavelength), step-wise down to those fluorochromes at the lower end of the spectrum … carafe for hamilton beach flexbrew