Flow cytometry staining buffer invitrogen
Web7. Wash the cells by adding 2 mL/tube of Flow Cytometry Staining Buffer. Centrifuge at 400–600 x g for 5 minutes. Discard supernatant. 8. Repeat Step 7. 9. Resuspend cells in an appropriate volume of Flow Cytometry Staining Buffer. 10. Analyze samples by flow cytometry, or if staining for intracellular targets, proceed with “Best Protocols: WebWash cells twice with Flow Cytometry Staining Buffer or equivalent. 7. Wash cells once with 1X Binding Buffer. 8. Resuspend cells in 1X Binding Buffer at 1-5 x 106 cells/mL. 9. Add 5 μL of fluorochrome-conjugated Annexin V to 100 μL of the cell suspension. 10. Incubate 10-15 minutes at room temperature.
Flow cytometry staining buffer invitrogen
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WebThe M3/38 monoclonal antibody specifically recognizes Galectin-3 (Gal-3 or gal3) which is also known as Galactose-specific lectin 3, Mac-2, MAC2, and Carbohydrate-binding protein 35 (CBP 35). Galectin-3 is an ~30-35 kDa protein that includes an N-terminal proline-rich tandem repeat domain as well as a C-terminal region with one carbohydrate recognition … WebSYTOX® Green stain a simple and quantitative single-step dead-cell indicator for use with fluorescence microscopes, fluorometers, fluorescence microplate readers, and flow cytometers (Figure 1). This dead-cell stain may be used in conjunction with blue- and red-fluorescent surface labels for multiparameter analyses.
WebA buffered saline solution containing fetal bovine serum and sodium azide (0.09%) as a preservative. This buffer can be used for antibody and cell dilution steps, as well as all … WebAdd 0.1-10 μg/ml of the primary labeled antibody. Dilutions, if necessary, should be made in FACS buffer. Incubate for at least 30 min at room temperature or 4°C in the dark. This step will require optimization. Wash the cells 3 times by centrifugation at 1500 rpm for 5 minutes and resuspend them in 200 μl to 1ml of ice cold FACS buffer*.
Webof Flow Cytometry Staining Buffer or buffer of choice. Tease apart into a single-cell suspension by pressing with the plunger of a 3-mL syringe. Alternatively, mash tissue … WebJun 3, 2024 · Flow cytometry is a lab technique used to look at individual cells in a sample of blood, semen, or bone marrow. Flow cytometry results can be used for cancer …
Web1 day ago · Co-culture and multi-parametric flow cytometry. C17 and NPC43 NPC cell lines were pre-treated with indicated doses of IDX and/or Cis for 24 h before coculturing with PBMCs via trans-wells (3 µm pore-size, Corning) for 3 days. For phenotypic surface staining, PBMCs were collected, washed and resuspended in FACS buffer (PBS, 0.5 % …
WebAll antibodies in this kit are compatible with the Intracellular Flow Cytometry Kit (Triton X-100) #51995 and can be used in a single staining mix on fixed and permeabilized cells. Prior to fixation and antibody incubation, we recommend adding a fixable viability dye such as the Ghost Dye Violet 510 Fixable Viability Dye #59863 to enable ... thomas merchtem new hollandWebR718 Mouse Anti-Human Myeloperoxidase. Multiparameter flow cytometric analysis of Myeloperoxidase expression in Human peripheral blood leucocyte populations. Human whole blood was treated with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) to remove erythrocytes and to fix leucocytes. The fixed leucocytes were permeabilized with BD … thomas meola mdWebAdd 2ml of 1X Red Blood Cell Lysis Buffer and incubate for 5-10 minutes at room temperature. Centrifuge at 350xg for 5 minutes and discard the supernatant. Wash cells … thomas menzingerWebSample preparation reagents for flow cytometry include cell surface staining, intracellular and transcription factor staining buffer sets, cell lysis assays, blocking reagents, and … uhip igloo coatWebProceed to analysis by flow cytometry. Staining of Fixed Cells for DNA Content Analysis by Flow Cytometry. 1. Obtain a single cell suspension. 2. Treat cells on ice for 30 minutes with 70-80% ice-cold ethanol. a. Ethanol fixation typically provides the most resolved histograms. However, this reagent has also been successfully used for DNA ... uhip guelphWebstaining. 1.4 6Prepare flow cytometry samples each containing ~ 1 × 10 cells in suspension. 1.5 Centrifuge the samples and decant the supernatant, leaving a pellet of cells in each sample tube. ™1.6 Add 0.5 mL of FxCycle PI/RNase Staining Solution stain to each flow cytometry sample, mix well. uhi phd feesuhi orkney archaeology