WebDec 29, 2011 · Expression of SV2A-EGFP in wild-type neurons reduces neurotransmitter release. We examined the effect of expressing SV2A-EGFP (SV2A with enhanced green fluorescent protein (EGFP) fused to the carboxy terminus) in wild-type autaptic cultures of hippocampal neurons as part of a larger study designed to assess the effects of … WebAcGFP1 is a monomeric green fluorescent protein with 94% homology to EGFP at the amino acid level. It has been widely validated as a fusion tag with a wide variety of …
EGFP :: Fluorescent Protein Database
WebAcGFP1 is a monomer, which makes it a superior alternative for fusion applications. EGFP has been reported to form dimers (Jain et al. 2001; Chen et al. 2002). Another alternative … WebStage 1 (eGFR of 90 or higher) indicates mild kidney damage, but your kidneys are working well. Stage 2 (eGFR between 60 and 89) indicates an increase in kidney damage from stage 1, but the kidneys continue to function well. Stage 3 (eGFR between 30 and 59) means you have decreased kidney function and may experience symptoms. how to document system architecture
2A self-cleaving peptide-based multi-gene expression system …
WebAug 3, 2024 · MDR1-EGFP-transduced variants were derived from WT cells by lentiviral transduction, using an MDR1-linker-EGFP vector. Localization, trafficking, and function of Pgp were compared in WT and MDR1-EGFP transduced cell lines. Primary cultures of rat BCECs and freshly isolated rat brain capillaries were used for comparison. WebJun 18, 2024 · Silencing of eGFP fluorescence was observed in both the maturation and elongation zones of roots. BMVCP5 mediated significant silencing of eGFP and TaPHO2 mRNA expression in roots at 14 and 21... Virus-induced gene silencing (VIGS) is a rapid and powerful method to evaluate gene function, especially for species like hexaploid wheat … WebOct 15, 2012 · Electrophoretic analysis of pEGFP - N1 - DTA digested with two enzymes with BglII and EcoRI. Lane 1, DNA marker (5 kb); lane 2, pEGFP-N1 fragment (top band) and DTA fragment (bottom band). Full size image Figure 3 Plasmid vector maps of pEGFP - N1 and pEGFP - N1 - DTA. A) Main elements of pEGFP-N1-DTA. learning to drive heavy equipment