WebPvuI-HF has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10128368. ... One unit is defined as the amount of enzyme required to digest 1 µg of … WebThis product was discontinued on 12/15/2024 and is replaced with the high fidelity version, PvuI-HF™ ( NEB #R3150 ). We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2024 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some ...
FastDigest PvuI - Thermo Fisher Scientific
WebThermo Scientific PvuI restriction enzyme recognizes CGAT^CG sites and cuts best at 37°C in R buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion. . Isoschizomers: … WebThis gel depicts the NEB 1kb ladder and a digestion of the plasmid pcDNA3.1(+) CircRNA Mini Vector with the following enzymes: PvuI-HF and XhoI. DNA digested with PvuI-HF single digest and PvuI-HF/ XhoI double digest for 2 hours. Gel: 0.8% agarose stained with 5 uL of ethidium bromide. Ran for 1.5 hours at 100 volts. I-HF / I scrim shield repair tape
PvuI (10 U/µL) - Thermo Fisher Scientific
WebDescription. Lambda DNA digested with PvuI, 0.7% agarose, 3 cleavage sites. conventional restriction endonucleases are a large collection of high quality restriction … WebThermo Scientific FastDigest PvuI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers. The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 … Thermo Scientific T4 DNA Polymerase is a template-dependent DNA polymerase … Thermo Scientific FastDigest PvuI is one of an advanced line of fast restriction … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes WebThermo Scientific™ FastDigest PvuI. Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one … scrimshire